A frog named Toughie, likely the last of his species, died quietly in his enclosure at the Atlanta Botanical Garden this week, reports the Atlanta Journal-Constitution.

“He will be missed by Garden staff and visitors alike,” the Garden posted on Facebook. The Rabbs’ fringe-limbed tree frog wasn’t even discovered until 2005 when scientists were attempting to rescue specimens of any amphibian they could before a deadly chytrid fungal infection hit Panama.

According to National Geographic, Toughie made it out of Panama, but it’s estimated that the chytrid fungus killed up to 85 percent of all amphibians left behind in his natural habitat.

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